SILVER NANOPARTICLE BIOSYNTHESIS USING FUSARIUM OXYSPORUM: A COMPARATIVE STUDY IN WHOLE CELL SYSTEMS WITH GROWING CELL SYSTEMS

Abstract

Biosynthesis of nanoparticles is a strong alternative for other methods of synthesis mainly due to its environmental friendly approach. It requires mild experimental conditions which can be varied easily to synthesize nanoparticles of desired properties. Micro organisms have very high potential for synthesizing nanoparticles with well demanded properties and applications. Here, we report the synthesis of silver nanoparticles using Fusarium oxysporum in the growing cell, whole cell, extracellular and intracellular systems. A detailed comparative study is done in the whole cell systems and growing cell systems by changing concentrations. The characterisation studies were conducted by Ultra Violet visible spectrophotometer, Transmission Electron Microscopy and by Energy Dispersive Spectroscopy. TEM images show that the silver nanoparticles are crystalline and fairly monodispersed in the case of whole cell systems
and polydispersed in the other case. Selected Area Electron Diffraction pattern shows that nanoparticles are crystalline. Energy Dispersive Spectroscopy spectra shows silver signal between 1 and 2 keV.